微生物溶解内蒙古褐煤影响因素及产物分析

西安科技大学 化学与化工学院,陕西 西安 710054

微生物; 溶煤产物; 红外光谱; 紫外光谱; 气相色谱-质谱联用仪

Influential factors and products of biosolubilization of Inner Mongolia lignite
YUE Zi-lin,LIU Xiang-rong*,ZHAO Shun-sheng,YANG Zai-wen,WANG Yi-fan,AN Zi-e

(College of Chemistry and Chemical Engineering,Xi'an University of Science and Technology,Xi'an 710054,China)

microorganism; biosolubilization product; IR; UV-VIS; GC-MS

DOI: 10.13800/j.cnki.xakjdxxb.2017.0216

备注

利用产碱假单胞菌、施氏假单胞菌、铜绿假单胞菌和粗毛栓菌4种微生物对内蒙古褐煤进行溶煤实验,探索了煤样粒度、氧化程度、溶煤时间和溶煤方式等因素对溶煤率的影响,并对溶煤产物进行了红外光谱(IR)、紫外光谱(UV-VIS)和气相色谱-质谱联用(GC-MS)分析。结果 发现,当煤样粒度为0.074~0.125 mm,氧化程度为10 mol/L硝酸浸泡处理48 h,溶煤时间为14 d和以摇瓶培养的方式进行溶煤时效果最好,此条件下铜绿假单胞菌的溶煤率最高,达62.61%.IR分析结果显示,残煤红外光谱图与氧化煤红外光谱图相比,羟基振动峰增强,芳烃结构振动峰减弱; UV-VIS分析结果显示,溶煤产物的主要成分为苯酚衍生物、醛类和醚类化合物; GC-MS分析结果显示,氧化煤经过微生物溶解以后,其结构发生变化,部分溶解为低分子量化合物。

Biosolubilization of Inner Mongolia lignite by Pseudomonas alcaligenes,Pseudomonas stuzeri,Pseudomonas aeruginosa and Trametes hirsute were studied.The grain size of coal sample,degree of oxidation,time of biosolubilising coal and the mode of biosolubilising coal were investigated,which influenced the percentage of biosolubilization.The coal biosolubilization products were characterized by UV-VIS,IR spectrum and GC-MS.The results showed that the percentage of biosolubilization was better when the coal sample size was 0.074~0.125 mm,oxidized by 10 mol/L nitric acid for 48 hours,biosolubilising time was 14 days and shaking culture was chosen,Pseudomonas aeruginosa was the highest,which was 62.61%.IR analysis showed the residual coals comparing with oxidized coal had stronger hydroxyl vibration peak and weaker aromatic vibration peak.UV-VIS analysis showed the main components of products are phenol derivatives,aldehydes and ethers.According to GC-MS analysis,the structure of oxidized coal was partly changed into low molecular weight compounds after the process of biosolubilization.