[1]聂文杰,王生全,侯晨涛,等.降解甲烷微生物的生物学特性研究[J].西安科技大学学报,2011,(05):530-533.
 NIE Wen-jie,WANG Sheng-quan,HOU Chen-tao,et al.Biological characteristics of methane-degradation bacterium[J].Journal of Xi'an University of Science and Technology,2011,(05):530-533.
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降解甲烷微生物的生物学特性研究()
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西安科技大学学报[ISSN:1672-9315/CN:61-1434/N]

卷:
期数:
2011年05期
页码:
530-533
栏目:
出版日期:
2011-09-29

文章信息/Info

Title:
Biological characteristics of methane-degradation bacterium
文章编号:
1672-9315(2011)05-0530-04
作者:
聂文杰王生全侯晨涛刘丹丹
西安科技大学 地质与环境学院,陕西 西安 710054
Author(s):
NIE Wen-jieWANG Sheng-quanHOU Chen-taoLIU Dan-dan
College of Geology and environment Engineering,Xi'an University of Science and Technology,Xi'an 710054,China
关键词:
瓦斯 降解甲烷微生物 16S rDNA测序 分离鉴定 生物学特性
Keywords:
gas methane-degradation bacterium 16S rDNA sequencing isolation and identification
分类号:
TD713.3;Q939.99
文献标志码:
A
摘要:
瓦斯是煤矿安全生产的最大隐患,利用微生物技术治理煤矿瓦斯的研究是目前国内外学者研究的热点。从垃圾填埋场的湿土中成功筛选出一种能降解甲烷的菌株,应用形态学观察及16S rDNA序列的同源性分析对该菌种进行了鉴定,同时研究了其培养条件及降解甲烷的性能。结果表明:该菌种属萎缩芽孢杆菌属(Bacillus atrophaeus),其最佳生长温度为32 ℃,pH值为6.5,氧气与甲烷体积比为2:3,在此条件下48 h内甲烷降解率接近50%.
Abstract:
Gas is the most dangerous in coal mine. At present, it is the focus of domestic and foreign studies to manage coal-mine gas by microbe. Methane-degradation bacterium was collected from refuse landfill of Xi'an Jiang Cun gutter. Identify the bcterium using morphological observation and 16S rDNA sequencing and comparison. The culturing and reaction conditions for methane utilization of this strain were investigated. The results indicated that the bacterium is Bacillus atrophaeus and the optimal temperature for the growth of the cultivation is 32 ℃ and medium initial pH 6.5, and the O2:CH4=2:3. Methane utilization of this strain in 48 hours was nearly 50% in this condition.

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备注/Memo

备注/Memo:
基金项目: 陕西省自然科学基金项目(SJ08-ZT08); 陕西省教育厅专项基金(09JK587); 陕西省教育厅项目(11JK0788) 通讯作者: 聂文杰(1981-),男,河南项城人,硕士,主要从事环境工程专业实验室教学与管理工作.
更新日期/Last Update: 2011-09-17