[1]岳子林,刘向荣*,赵顺省,等.微生物溶解内蒙古褐煤影响因素及产物分析[J].西安科技大学学报,2017,(02):251-259.[doi:10.13800/j.cnki.xakjdxxb.2017.0216]
 YUE Zi-lin,LIU Xiang-rong*,ZHAO Shun-sheng,et al.Influential factors and products of biosolubilization of Inner Mongolia lignite[J].Journal of Xi'an University of Science and Technology,2017,(02):251-259.[doi:10.13800/j.cnki.xakjdxxb.2017.0216]
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微生物溶解内蒙古褐煤影响因素及产物分析(/HTML)
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西安科技大学学报[ISSN:1672-9315/CN:61-1434/N]

卷:
期数:
2017年02期
页码:
251-259
栏目:
出版日期:
2017-03-30

文章信息/Info

Title:
Influential factors and products of biosolubilization of Inner Mongolia lignite
文章编号:
1672-9315(2017)02-0251-09
作者:
岳子林刘向荣*赵顺省杨再文王一凡安子锷
西安科技大学 化学与化工学院,陕西 西安 710054
Author(s):
YUE Zi-linLIU Xiang-rong*ZHAO Shun-shengYANG Zai-wenWANG Yi-fanAN Zi-e
College of Chemistry and Chemical Engineering,Xi'an University of Science and Technology,Xi'an 710054,China
关键词:
微生物 溶煤产物 红外光谱 紫外光谱 气相色谱-质谱联用仪
Keywords:
Key words:microorganism biosolubilization product IR UV-VIS GC-MS
分类号:
TQ 536.1
DOI:
10.13800/j.cnki.xakjdxxb.2017.0216
文献标志码:
A
摘要:
利用产碱假单胞菌、施氏假单胞菌、铜绿假单胞菌和粗毛栓菌4种微生物对内蒙古褐煤进行溶煤实验,探索了煤样粒度、氧化程度、溶煤时间和溶煤方式等因素对溶煤率的影响,并对溶煤产物进行了红外光谱(IR)、紫外光谱(UV-VIS)和气相色谱-质谱联用(GC-MS)分析。结果发现,当煤样粒度为0.074~0.125 mm,氧化程度为10 mol/L硝酸浸泡处理48 h,溶煤时间为14 d和以摇瓶培养的方式进行溶煤时效果最好,此条件下铜绿假单胞菌的溶煤率最高,达62.61%.IR分析结果显示,残煤红外光谱图与氧化煤红外光谱图相比,羟基振动峰增强,芳烃结构振动峰减弱; UV-VIS分析结果显示,溶煤产物的主要成分为苯酚衍生物、醛类和醚类化合物; GC-MS分析结果显示,氧化煤经过微生物溶解以后,其结构发生变化,部分溶解为低分子量化合物。
Abstract:
Abstract:Biosolubilization of Inner Mongolia lignite by Pseudomonas alcaligenes,Pseudomonas stuzeri,Pseudomonas aeruginosa and Trametes hirsute were studied.The grain size of coal sample,degree of oxidation,time of biosolubilising coal and the mode of biosolubilising coal were investigated,which influenced the percentage of biosolubilization.The coal biosolubilization products were characterized by UV-VIS,IR spectrum and GC-MS.The results showed that the percentage of biosolubilization was better when the coal sample size was 0.074~0.125 mm,oxidized by 10 mol/L nitric acid for 48 hours,biosolubilising time was 14 days and shaking culture was chosen,Pseudomonas aeruginosa was the highest,which was 62.61%.IR analysis showed the residual coals comparing with oxidized coal had stronger hydroxyl vibration peak and weaker aromatic vibration peak.UV-VIS analysis showed the main components of products are phenol derivatives,aldehydes and ethers.According to GC-MS analysis,the structure of oxidized coal was partly changed into low molecular weight compounds after the process of biosolubilization.

参考文献/References:

[1] 任海宁.褐煤深加工技术及发展前景[J].山东化工,2016,45(2):66-69. REN Hai-ning.Processing technology and development prospect of lignite[J].Shandong Chemical Industry,2016,45(2):66-69.
[2] 李恩利,高建国,崔红梅,等.中国褐煤提质项目风险分析[J].煤炭经济研究,2009(12):25-26. LI En-li,GAO Jian-guo,CUI Hong-mei,et al.Lignite mention qualitative project risk analysis in our country[J].Coal Economic Research,2009(12):25-26.
[3] 王 琴,傅 霖,辛明秀.微生物降解煤的研究及其应用[J].煤炭加工与综合利用,2009(3):38-41. WANG Qin,FU Lin,XIN Ming-xiu.Research and application of the degradable coal converted by microorganism[J].Coal Processing and Comprehensive Utilization,2009(3):38-41.
[4] XIAO Lei,YAO Jing-hua,WAN Yong-zhou,et al.Briquetting mechanism and parameters optimization of the lignite/biomass[J].Journal of China University of Mining and Technology,2010,39(3):352-356.
[5]YUAN Hong-li,YANG Jin-shui,WANG Feng-qin,et al.The prospect of microbial sustainable utilization of lignite[J].World Sci-tech R & D,2002,24(3):13-17.
[6]YAO Jing-hua,XIAO Lei,WANG Li-qiang.Separation and analysis of lignite bioconversion products[J].International Journal of Mining Science and Technology,2012,22(4):529-532.
[7]Reiss J.Studies on the solubilization of German coal by fungi[J].Applied Microbiology,1992,37(6):830-832.
[8]Oboirien B O,Stephanie Burton,Donald A Cowan,et al.The effect of the particulate phase on coal biosolubilization mediated by trichoderma atroviride in a slurry bioreactor[J].Fuel Processing Technology,2008,89(2):123-130.
[9]Laborda F,Monistrol I F,Luna N,et al.Processes of liquefaction/solubilization of Spanish coals by microorganisms[J].Applied Microbiology and Biotechnology,1999,52(1):49-56.
[10]Cohen M S,Gabriele P D.Degradation of coal by the fungi polyporus versicolor and poria monticola[J].Applied and Environmental Microbiology,1982,44(1):23-27.
[11]王龙贵,张明旭,欧泽深,等.白腐真菌对煤炭的降解转化试验[J].煤炭学报,2006,31(2):241-244. WANG Long-gui,ZHANG Ming-xu,OU Ze-shen,et al.Experimental of coal biodegradation by white rot fungus[J].Journal of China Coal Society,2006,31(2):241-244.
[12]石开仪,陶秀祥,尹苏东,等.真菌固定化在褐煤转化中的应用[J].煤炭工程,2008(1):78-80. SHI Kai-yi,TAO Xiu-xiang,YIN Su-dong,et al.The Application of fungi immobility in lignite bio-solubilization[J].Coal Engineering,2008(1):78-80.
[13]Faison B D,Woodward C A,Bean R M.Microbial solubilization of a preoxidized subbituminous coal[J].Applied Biochemistry and Biotechnology,1990,24(1):831-841.
[14]Charles D Scott,Gerald W Strandberg,Susan N Lewis.Microbial solubilization of coal[J].Bloteehnology Progcess,1986,2(3):131-139.
[15]Ghani M J,Rajoka M I,Kalsoom Akhtar.Investigations in Fungal solubilization of coal:mechanisms and significance[J].Biotechnology and Bioprocess Engineering,2015,20(4):634-642.
[16]Paul J Dodson,Christine S Evans,Pat J Harvey,et al.Production and properties of an extracellular peroxidase from Coriolus versicolor which catalyses Cα-Cβ cleavage in a lignin model compound[J].FEMS Microbial Lett,1987,42(1):17-22.
[17]Hofrichter M,Bublitz F,Fritsche W.Fungal attack on coal Ⅱ:Solubilization of low-rank coal by filamentous fungi[J].Fuel Processing Technology,1997,52(1-3):55-64.
[18]Fakoussa R M,Hofrichter M.Microbiology and biotechnology of coal degradation[J].Applied Microbiol Biotechnology,1999,52(1):25-40.
[19]Hofrichter M,Steinbuchel A.Biopolymers Volume 1:Lignin,Humic Substances and Coal[M].Oxford:Wiley-Blackwell,2001.
[20]Fuchtenbusch B,Steinbuchel A.Biosynthesis of polyhydroxyalkanaotes from low-rank coal liquefaction products by Pseudomonas oleovorans and Rhodococcus rubber[J].Appl Microbiol Biotechnol,1999,52:91-95.
[21]TAO Xiu-xiang,PAN Lan-yang,SHI Kai-yi,et al.Bio-solubilization of Chinese lignite Ⅰ:extra-cellular protein analysis[J].Mining Science and Technology,2009,19(3):358-362.
[22]YIN Su-dong,TAO Xiu-xiang,SHI Kai-yi.Bio-solubilization of Chinese lignite:protein adsorption onto the lignite surface[J].Mining Science and Technology,2009,19(3):363-368.
[23]YIN Su-dong,TAO Xiu-xiang,SHI Kai-yi.The role of surfactants in coal bio-solubilization[J].Fuel Processing Technology,2011,92(8):1 554-1 559.
[24]TANG Hui-ying,YAN Dan,ZHANG Qing-Zhe,et al.Microcalorimetric investigation of four bioactive extracts from cornu cervi pantotrichumon intestinal diagnostic flora growth[J].Acta Physico-chimica Sinica,2010,26(5):1 442-1 447.
[25]Rizwan Haider,Muhammad A Ghauri,John R Sanfilipo,et al.Fungal degradation of coal as a pretreatment for methane production[J].Fuel,2013,104:717-725.
[26]GAO Tong-guo,JIANG Feng,YANG Jin-shui,et al.Biodegradation of leonardite by an alhali-producing bacterial community and characterization of the degraded products[J].Applied Microbiology and Biotechnology,2012,93(6):2 581-2 590.
[27]HUANG Zai-xing,Christiane Liers,Rene Ullrich,et al.Depolymerization and solubilization of chemically pretreated powder river basin subbituminous coal by manganese peroxidase(MnP)From Bjerkandera adusta[J].Fuel Processing Technology,2013,112:295-301.
[28]SHI Kai-yi,TAO Xiu-xiang,YIN Su-dong,et al.Bio-liquefaction of Fushun lignite:characterization of newly isolated lignite liquefying fungus and liquefaction products[J].Procedia Earth and Planetary Science,2009,1(1):627-633.
[29]YIN Su-dong,TAO Xiu-xiang,SHI Kai-yi,et al.Biosolubilisation of Chinese lignite[J].Energy,2009,34(6):775-781.
[30]Irena Romanowska,Bartosz Strzelecki,Stanis aw Bielecki.Biosolubilization of Polish brown coal by Gordonia alkanivorans S7 and Bacillus mycoides NS1020[J].Fuel Processing Technology,2015,131:430-436.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金(21373158); 陕西科技厅中澳合作项目(2013KW17) 通讯作者:刘向荣(1966-),女,陕西西安人,教授, E-mail:liuxiangrongxk@163.com
更新日期/Last Update: 1900-01-01